Fig. (4) dD/dF ratios (the primary X and Y axis) and dF values (the secondary Y axis [right] and the secondary X axis shown [top] by ultrafine dotted lines) for binding of VCP, tVCP, Cur, and C3 on PS. dD/dF ratios at 30 min interval are shown by solid black bars whereas final dD/dF ratios are shown by hatched bars. The dF30 values are shown by a gradient black bar whereas the dFfin values are shown by a black hatched line with gradient black dots (). The dF and dD values shown indicate the average values obtained from the two experiments unless specified in the Table 1.