Fig. (5) dD/dF ratios (the primary X [bottom] and Y axis [black; left]) and dF values (the secondary Y axis [right] and X axis [Top] are shown by ultrafine dotted lines at the top) for the binding of VCP, tVCP, Cur and C3 on the gold surface are as shown in this figure. dD/dF ratios at 30 min interval are shown by solid black bars whereas final dD/dF ratios are shown by hatched bars. The dF₃₀ values are shown by a gradient black bar whereas the final dF values are shown by the black line with gradient black dots (). The dD/dF ratios and dF values indicate average of the values obtained from two experiments unless specified in the Table 1.