Fig. (2) The glutamine 324 residue of Tip60 is required for the interaction with FE65 and its HAT Activity A) After immunoprecipitating the transfected GFP Tip60 WT, N324A, N342A with monoclonal GFP Ab, the precipitant was immuno blotted with FE65 Ab, B) Acetylated-lysine Ab, or C) Tip60 Ab. D) In parallel, HAT activity of purified Tip60 WT, N324A, or N342A expressed in HEK 293 cell were compared with ELISA method in arbitrary units. For the detail, see the material and method. * P < 0.05 significance versus to each other.