Table 2: qRT-PCR validation of array response. qRT-PCR was conducted using custom designed primer plates to validate the response of the miRNA arrays. Statistically significant differentially expressed miRNA with corresponding p-values and fold changes (FC) which were found to respond at all doses of α-particle radiation tested (0.5, 1.0, and 1.5Gy). Statistical cut-off at p ≤ 0.05
Cell-Type |
µRNA |
0.5 Gy FC |
SEM |
1.0 Gy FC |
SEM |
1.5 Gy FC |
SEM |
A549 |
hsa-miR-486-5p |
2.02 |
0.30 |
1.86 |
0.15 |
2.21 |
0.21 |
|
|
|
|
|
|
|
|
HFL-1 |
hsa-miR-675 |
2.06 |
0.25 |
2.10 |
0.15 |
2.30 |
0.22 |
|
hsa-miR-663 |
2.10 |
0.26 |
2.01 |
0.14 |
2.14 |
0.31 |
|
hsa-miR-560:9.1 |
2.15 |
0.31 |
2.08 |
0.19 |
2.2 |
0.14 |
|
|
|
|
|
|
|
|
THP-1 |
hsa-miR-708 |
2.00 |
0.26 |
2.20 |
0.16 |
2.60 |
0.16 |