Fig. (3) Northern blot analysis of steady-state APP and 7s mRNA levels after treatment with increasing concentrations of S-MIU and 20mM L-NAME in the presence or absence of NGF. (A) APP695 mRNA levels: NGF (50 ng/mL) alone and 1.5 or 2.0 mM S-MIU with and without NGF significantly (* p < 0.05; ** p < 0.01; *** p < 0.001) decreased APP695 mRNA levels as compared to control. (B) APP751/770 mRNA levels: NGF alone, and the two concentrations of S-MIU in the presence or absence of NGF significantly (** p < 0.01; *** p < 0.001) decrease in APP751/770 mRNA levels as compared to control. Data are presented as the mean ± SEM and represent 6 independent experiments. Statistical significance was determined by a one-way ANOVA followed by the Dunnett test.