Fig. (1) Schematic representation of the experimental design and the quantitative proteomics analyses showing biological and technical replicates. Following tianma treatment (batches B-I (5 rats) and B-II (5 rats): +T; control = B-I (5 rats) and B-II (5 rats): -T) and aortic tissue lysis, protein extracts were acetone precipitated and quantified. These were then run in SDS-PAGE and subsequently digested. The quantitative proteomics analyses of aortic tissue lysates were performed by labeling with multi-plex isobaric tags (114, 115, 116 and 117) for relative and absolute quantification (iTRAQ) followed by Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC)-based fractionation, and liquid chromatography coupled with tandem mass spectrometry (LCMS/ MS)-based multidimensional protein identification technology. The obtained data was analyzed using ProteinPilot software and validated by quantitative western blots. Finally, proteins were functionally classified into various subgroups.