Fig. (3) Tianma enhanced ACh-induced vasorelaxation in endothelium-intact rat thoracic arterial rings (II). Analysis of ACh induced
relaxations in PE (10-6 M) pre-contracted isolated endothelium-intact
arterial rings from control and tianma-treated rats. One-year-old
rats were treated with or without tianma for three months at
~2.5g/kg/day and the thoracic aortas were isolated. The endothelium-intact arterial rings were first pre-contracted by PE (10-6 M).
Increasing concentrations of ACh were added and then the percentage
of ACh-induced relaxation to PE (10-6 M) contraction was
measured as described in the materials and methods. Data points
represent means ± SD of measurements in 8 arterial rings from 5
rats of each group (*P < 0.05 compared with the control group).