Fig. (5) Simulated 2D gel presentation of rat thoracic aorta-derived quantified proteins. MW and pI values generated from JVirGel (http://www.jvirgel.de/). The proteins identified by LC-MS/MS were uploaded onto JVirGel, online software used to create a 2D gel image. This image confirmed that the tissue lysis performed was adequate and the entire proteome within cells was extracted.