Fig. (2) Overexpression and purification profile of MsmNDK and MsmNDK-H117Q. A. Coomassie blue stained SDS-PAGE profile of overexpressed MsmNDK (2) and MsmNDK-H117Q (4). Lanes 1 and 3, respective uninduced samples. Arrow head indicates the overexpressed or purified protein. B. SDS–PAGE of recombinant purified MsmNDK wild type (1) and MsmNDK-H117Q (2) proteins; lane M represents molecular weight markers. C. Far-UV circular dichroism (CD) spectrum of MsmNDK and MsmNDK-H117Q.