Fig. (5) NTP synthesising activity of MsmNDK and MsmNDK-H117Q. A. PEI-TLC profile of [γ-32P]-ATP alone (lane 1), and in the presence of [γ-32P]-ATP: GDP + MsmNDK (lane 2), GDP + MsmNDK-H117Q (lane 3), CDP + MsmNDK (lane 4), and CDP + MsmNDK-H117Q (lane 5). B. Bar graph representing quantitation of unhydrolysed [γ-32P]-ATP. The positions of ATP, CTP, and GTP were identified using the Rf values for these NTPs in the solvent system, 0.75M KH2PO4. Assay was repeated independently three times for each sample. Counts per minute (CPM) were recorded and standard deviations were calculated.