Fig. (2) Effects of xanthohumol on the expression of ANXA1 in three different glioblastoma T986, U87-MG and 4343-MG.cell lines: The three cell lines were incubated in the presence and in the absence of xanthohumol 20µM at different time points (0-48 h). The expression of ANXA1 was analyzed by Western blotting, using a polyclonal anti-ANXA1 and normalizing the amount of protein analyzed by an antibody anti-β-actin.