Fig. (5) Effects of siRNAs directed against ANXA1 expression da togliere in T98G cells. T98G cells were transfected with oligofectamina containing concentrations of 100nM of small interference oligonucleotides directed against ANXA1 and scrambled control. After 48 hours of transfection, the cells were treated with xanthohumol 20µ for 24 h. Subsequently 30μg of protein were analyzed by Western blotting using a polyclonal antibody anti ANXA1. Data are shown as optical density, mean ± SEM of three representative experiments. Bonferroni test (*** P <0.001 vs CTR, ° ° ° P <0.001 vs scrambled xanthohumol and xanthohumol +).