Fig. (6) (A) Effect of siRNA-ANXA1 on apoptosis of T98G cells analyzed by staining with propidium iodide (PI) as an expression of hypodiploid nuclei. The cells were transfected for 48 hours with siRNA-ANXA1 (100nM) and siRNA-scrambled (SK) control and subsequently treated with xanthohumol for 24 hours. (B) Effect of siRNA-ANXA1 activation of caspase-3 in T98G cells measured by fluorimetric assay. The cells were transfected for 48 hours with si-RNA-ANXA1 (100nM) and subsequently treated with xanthohumol for 8-24-48 hours and then analyzed by fluorometric assay specific for caspase-3. Data are shown as mean ± SEM of three representative experiments representative. Bonferroni test (*** P <0.001 vs. control cells; § § § P <0.001 vs. control cells and cells transfected with SK, ° ° P <0.01 vs. cells treated with XH and XH + SK).