Fig. (1)
NS5B D 8 mutant is not affected by NS5A in the synthesis
of the full length RNA. NS5B wt (0.9µM) or NS5B D8 (0.2
µM) was incubated with NS5A (26 nM, 260 pM, 2.6 pM) under the
conditions previously described [9] with the LTMOA template (0.5
µM) for 60 min. The products of the reaction were separated on a
PAGE-UREA gel, then exposed on a phosphorimager cassette and
scanned.