Fig. (2) Redox conditions affect reductide uptake and intracellular fluorescence.Live cell time-lapse confocal microscopic images (60X) of BJ fibroblasts incubated with reductide are shown. Cells were seeded into 4-chamber glass cover slides and allowed to attach overnight. The following day, the plates were pretreated with either CDNB 25 µM or NAC 4 mM for 30 minutes prior to washing with PBS and incubating with reductide 4 µM in normal media. TAMRA emission images are shown in red, FAM images are shown in green, and DAPI images are shown in blue. Arrows indicate exocytic vesicles containing FAM but not TAMRA.