Fig. (6) Cellular uptake of reductide is affected by redox state.To compare the effects of cellular redox modification prior to incubation with reductide with modification after incubation with reductide, we incubated BJ fibroblasts first with reductide 1 µM for one hour followed by washing with PBS and subsequent treatment with redox modifying agents (NAC or H₂O₂) for 60 minutes. Plate reader fluorescence results for cells treated with redox modifying agents first followed by reductide incubation (black bars) are shown in comparison with cells first incubated with reductide and afterward treated with redox modifying agents (gray bars).