Table 1: Selected Results from Testing a Library of 84 Redox-active Compounds for Effect on Reductide

Compound name Class % change from control P value comparison with control
Ethoxyquin Nonphenolic antioxidant 155.4 9.5E-05
Seratrodast Quinone antioxidant 134.9 9.1E-05
Retinyl palmitate Radical scavenger 123.5 2.5E-03
Idebenone Quinone antioxidant 120.8 3.3E-02
-carotene Radical scavenger 111.1 3.8E-04
Ebselen GSH peroxidase mimetic 77.2 0.00023
Cumene hydroperoxide Aryl hydroperoxide -30.8 1.7E-03
N-Ethylmaleimide Thiol trap -71.7 0.00001

BJ fibroblasts were seeded into a 96-well plate at a density of 4,000 cells per well and allowed to attach overnight in preparation for incubation with 50 µM of redox modifying compounds dissolved in cell media for 24 hours. Cells were subsequently washed with PBS and incubated with reductide 1.5 µM dissolved in cell media for four hours. FAM signal was assayed in a plate reader. The redox modifying compounds were obtained as an 84 compound library from Enzo Life Sciences. Percentage change in reductide signal in comparison with vehicle treated cells are shown for a subset of the redox modifying compounds.