Fig. (2) Tyrosine 327 phosphorylation of Tip60 promoted binding with FE65 and inhibited HAT activity. (A) Effect of tyrosine phosphorylation on the protein interaction with FE65. EGFP TIP60 WT, Y327F, and Y345F in HEK293 lysates were immunoprecipitated with anti-GFP and immunoblotted with FE65 antibody (above), anti-acetylated lysine (middle), or anti-TIP60 (bottom). (B) Quantification of HAT activity of EGFP Tip60 WT, Y327F, or Y345F by ELISA (the number is an artificial optical density. * means the statistical significance.