Fig. (2) Tyrosine 327 phosphorylation of Tip60 promoted binding
with FE65 and inhibited HAT activity. (A) Effect of tyrosine phosphorylation
on the protein interaction with FE65. EGFP TIP60 WT,
Y327F, and Y345F in HEK293 lysates were immunoprecipitated
with anti-GFP and immunoblotted with FE65 antibody (above),
anti-acetylated lysine (middle), or anti-TIP60 (bottom). (B) Quantification
of HAT activity of EGFP Tip60 WT, Y327F, or Y345F by
ELISA (the number is an artificial optical density. * means the
statistical significance.