The CCA-end of P-tRNA Contacts Both the Human RPL36AL and the A-site Bound Translation Termination Factor eRF1 at the Peptidyl Transferase Center of the Human 80S Ribosome

Fig. (8) Reaction scheme of the Schiff base formation and its in situ reduction with sodium borohydride (NaBH4) or sodium cyanoborohydride (NaBH3CN). With both reducing agents, the overall crosslinking reaction is rate-limited by the formation of the imminium moiety, not by its in situ reduction.