Figure 1: Development of the pGEX-6p-1-PTEN expression vector. (A) PCR products of PTEN encoding region. (B) Map of the pGEX-6p-1- PTEN expression construction and the orientation of the PTEN insertion fragment. (C) Restriction enzyme digestion with BamHI and EcoRI (lane 2-7) of six different clones before transforming into competent BL21 E.coli. The blank empty pGEX-6p-1 vector was used as the negative control (lane 1). DNA Marker (lane M).