Fig. (4) Purification and identification of the YheA protein in Staphylococcus aureus.The YheA protein is expressed in both planktonic growth and biofilm. The shaded squares show the fractions where the YheA protein was identified. A. Size-exclusion chromatography (SEC) of the total protein extract. The dotted black line shows the behavior of the recombinant YheA protein in the SEC. B. Anion-exchange chromatography (AEC) of the YheA-positive SEC fractions (14 and 15). Discontinuous black line indicates the NaCl gradient.