Fig. (1) Resistance of isolated kanMX4-inserted mutants to 2-DOG. Cells were grown in 2% YPGal medium at 30°C for 18 h and subjected to a spot test as described in MATERIALS AND METHODS. Sample of 10-fold serially diluted culture were spotted onto 2% YPD, 2% YPGal, 2% YPXyl, 2% YPGal containing 0.01% 2-DOG and 2% YPXyl containing 0.01% 2-DOG, plates were incubated at 30°C for 48 h.