Table 1: Primers used in this study.

Name Sequence Remark
bleF 5’-TCTGTACAGACGCGTGTACG-3’ Primer for ble amplification
bleR 5’-AACTAATTACATGATATCGA-3’ Primer for ble amplification
kanMX-KpnI5’ 5’-GATGGTACCCAGCTGAAGCTTCGTACGC-3’ Primer for kanMX4 amplification
kanMX-KpnI3’ 5’-CATGGTACCGCATAGGCCACTAGTGGATCTG-3’ Primer for kanMX4 amplification
kanMX-100-IV 5’-AGGAGGGTATTCTGGGC-3’ a
kanMX-1381-IV 5’-TGCGAAGTTAAGTGCGC-3’ a
kanMX-322-IV 5’-TGCTCGCAGGTCTGCAGCGAGGA-3’ a
kanMX-216-IV 5’-ACGGGCGACAGTCACATCATGCC-3’ a
kanMX-1135-IV 5’-AGTCGGAATCGCAGACCGATACC-3’ a
kanMX-1485-IV 5’-GGTCGCTATACTGCTGTCGATTC-3’ a
mig1-F 5’-ACAACCAACTATCCCGGTGC-3’ b
mig1-R 5’-AGAGATGGCATCATCAGTTCGT-3’ b
wtm2-F 5’-GCAGCAGGAATAACAGGACC-3’ b
wtm2-R 5’-TTTTCTCTGGGACAGAGTGT-3’ b
dfg16-F 5’-GGACAGAAAAGTCACGCAGC-3’ b
dfg16-R 5’-TGAGTTCTGCAACGCAGTCT-3’ b
ctm1-F 5’-TCGCTAAGTCATGCTGCTCC-3’ b
ctm1-R 5’-GTTGGAGTAGGTGGGTTCGG-3’ b
dig1-F 5’-CGAATTGAAGGCCACTTCGT-3’ b
dig1-R 5’-TGGCCGTGACCAAAGAAAGG-3’ b
msn5-F 5’-GGCCATGGTAGACCAACGAG-3’ b
msn5-R 5’-CGAACCTTTTCCCCCGGTAT-3’ b
alg10-F 5’-GATGCGTAAGTTCCGCCTGA-3’ b
alg10-R 5’-GAATCGCGCAAGTTGAGAGT-3’ b
nnk1-F 5’-TGGACTTGATAACGGCACCT-3’ b
nnk1-R 5’-ACCTAGATGAGTGGATGAGTGGA-3’ b
zip2-F 5’-TCGAAGGATTCCGTCTGCATT-3’ b
zip2-R 5’-CTCCTATCTAAAGGGCGAGGC-3’ b
rgi2-F 5’-ACAGTGCAGGTAGAAAAACCAT-3’ b
rgi2-R 5’-GTTGCATCATTTGAGGTGCAG-3’ b
mdj2-F 5’-ATGCAACCGCTCAGACAAGA-3’ b
mdj2-R 5’-CTCTGTTGGGACAGGTTCAGT-3’ b
arg3-F 5’-AGGAGCTTGGAATAACCGCC-3’ b
arg3-R 5’-GCACCAGACAGCCAATTTGA-3’ b

aPrimers for amplification and sequencing of flanking regions of inserted kanMX4
bPrimers for amplification of a corresponding gene to complement the kanMX4-inserted mutation.