Table 1: Conditions used for thrombin preparation in the study.

Test Factors Method Conditions
- - 0.25 M CaCl2 Volume (ml) RVV-X
(mcg)
0.85% NaCl Final Extraction Volume (ml) Incubation Time
(hours)
1) RVV-X M1.0 3 - 25 2
M2.0 3 50 25 2
2) 0.25 M CaCl2 M2.0 3 50 25 2
    Volume M2.1 1.5 50 25 2
3) 0.85% NaCl Final M2.0 3 50 25 2
    Extraction Volume M2.2 3 50 10 2
4) Incubation Time M2.2 3 50 10 2
- M2.3 3 50 10 -