Table 1: Comparison of main properties for three presented formats of immunochromatographic assays with indirect labeling.

Formats Stepwise ICA ICA when all Reagents are applied to the Test Strip ICA when Antibodies are in Solution
Advantages Assays steps do not affect each other Minimal limit of detection Pre-incubation is combined with sample dilution
Limitations Longer assay
More laborious manipulations
Influence of the test strip geometry and the mode of reagents deposition on the assay results Loss of sensitivity