Table 1: Comparison of main properties for three presented formats of immunochromatographic assays with indirect labeling.

Formats	Stepwise ICA	ICA when all Reagents are applied to the Test Strip	ICA when Antibodies are in Solution
Advantages	Assays steps do not affect each other	Minimal limit of detection	Pre-incubation is combined with sample dilution
Limitations	Longer assay More laborious manipulations	Influence of the test strip geometry and the mode of reagents deposition on the assay results	Loss of sensitivity