Table 1: Application of CRISPR/Cas9 based genome editing system in cereal crops. Details on the name of the cereal crop, type of study, name of the promoter, method of transformation, and name of the gene-edited are added with respective references.

Name of the Plant	Type of Study	Cas9 Promoter	sgRNA Promoter	Method of Delivery	Target Gene	References
Rice	Functional genomics	CaMV 35S	U6	Agrobacterium-mediated	OsSWEET14 and OsSWEET11	[31]
	Site-directed mutagenesis	CaMV 35S	OsU6-2	Agrobacterium-mediated	OsMYB1	[32]
	Site-directed mutagenesis	CaMV 35S	U3	Agrobacterium-mediated	OsPDS, OsBADH, Os02g23823 and OsMPK2	[22]
	Site-directed mutagenesis	CaMV 35S	U3 or U6	Protoplast transformation	MPK5	[17]
	Site-directed mutagenesis	CaMV 35S	OsU6-2	Agrobacterium-mediated	ROC5, SPP and YSA	[34]
	Gene editing	CaMV 35S	U6	Agrobacterium-mediated	OsPDS, OsPMS3, OsEPSPS, OsDERF1, OsMSH1, OsMYB5, OsMYB1, OsROC5, OsSPP and OsYSA**	[35]
	Genome editing	2 × 35S	U6	Agrobacterium- mediated	TaLOX2	[22]
	Multiplex editing capability with the endogenous tRNA	UBI	OsU3	Agrobacterium- mediated	OsMPKs	[33]
	Multiplex genome editing in monocot and dicot plants	UBI/35S	OsU3/U6	Agrobacterium- mediated	46 genomic targets	[18]
	Deletions and heritable small genetic changes induced	UBI	OsU6	Agrobacterium- mediated	OsSWEET11, and OsSWEET14	[36]
	Functional studies	OsUBI	OsU6, OsU3, and TaU3	Agrobacterium-mediated	GW2, GW5, and TGW6	[37]
	Functional studies	35S	U3	Agrobacterium-mediated	OsCYP97A4, OsDSM2, OsCCD4a, OsCCD4b, and OsCCD7	[38]
	Knock-out	ZmUBI	U6	Agrobacterium-mediated	Gn1a, DEP1, GS3, and GLW2	[39]
	Functional genomics	CaMV 35S	U6	Agrobacterium-mediated	OsRAV2	[40]
	Knock-out	ZmUBI	U3	Agrobacterium-mediated	ALS	[41]
	Knock-out	pHUN411–C3C5	U3	Protoplast transformation	EPSPS	[42]
	Knock-out	ZmUBI	U3 or U6	Agrobacterium-mediated	TMS5	[36]
	Functional genomics	2 × P35S	U6	Agrobacterium-mediated	ALS	[41]
	Knock-out	ZmUBI	U6	Agrobacterium-mediated	OsERF922	[43]
	Knock-out	pCXUN	U3	Agrobacterium-mediated	SBEI and SBEIIb	[44]
	Knock-out	ZmUBI	U6, U3	Agrobacterium-mediated	OsNramp5	[45]
	Functional studies	CaMV 35S	U6	Agrobacterium-mediated	OsAnn3	[46]
	Knock-out	OsUBI	U3	Agrobacterium-mediated	OsCCD7	[47]
	Knock-out	pZH	U3 and U6	Agrobacterium-mediated	OsFAD2-1	[48]
	Knock-out	UBI-1	U6	Agrobacterium-mediated	OsACC-T1OsALS-T1, OsCDC48-T3, OsDEP1-T1, OsDEP1-T2, and OsNRT1.1B-T1	[49]
	Knock-out	ZmUBI	U6	Agrobacterium-mediated	GS3, and Gn1a	[50]
	Functional studies	CaMV 35S	U3	Agrobacterium-mediated	GS9	[51]
	Functional studies	35S	U6	Agrobacterium-mediated	Bsrk1	[52]
	Knock-out	ZmUBI	U6	Agrobacterium-mediated	SAPK2	[53]
	Knock-out	ZmUBI	U6	Agrobacterium-mediated	elF4G	[54]
	Knock-out	CaMV 35S	U6	Agrobacterium-mediated	Waxy	[55]
	Knock-out	PUBI-H	U6	Agrobacterium-mediated	OsRR22	[56]
	Knock-out	CaMV 35S	U6	Agrobacterium-mediated	Wx	[57]
	Knock-out	CaMV 35S	U6	Agrobacterium-mediated	ISA1	[58]
Wheat	Site-directed mutagenesis	CaMV 35S	U6	Agrobacterium-mediated	Inox and PDS	[59]
	Site-directed mutagenesis	CaMV 35S	U6	Protoplast transformation	MLO	[11]
	Site-directed mutagenesis	CaMV 35S	U3 or U6	Particle bombardment	TaMLO-A1, TaMLO-B1 and TaMLO-D1	[60]
	Genome editing in wheat through transient expression	UBI	TaU6	Particle bombardment	immature embryos	[61]
	Knock-out	-	-	Protoplast transformation	TaGW2(A1, -B1 and D1)	[62]
	Gene editing	35S	U6	Biolistic	TaGASR7	[63]
	Functional genomics	UBI	U6 and U3	Agrobacterium-mediated	TaPDS	[64]
	Functional genomics	-	TaU6	Agrobacterium-mediated	TaDREB2 and TaERF3	[65]
	Knock-out	2×35S	U6	Biolostic	TdGASR7	[66]
	Genome editing	ZmUBI	U6	Agrobacterium-mediated	TaCKX2-1, TaGLW7, TaGW2, and TaGW8	[67]
Maize	Targeted mutagenesis	CaMV 35S	ZmU3	Agrobacterium-mediated	ZmIPK	[25]
	Targeted mutagenesis	UBI	ZmU6	Agrobacterium-mediated	ZmLIG1, ZmM26, Zm45, and ZmALS1	[68]
	Targeted mutagenesis	UBI	U6	Particle bombardment	ALS2	[69]
	Genome editing	2×35S	U3	Agrobacterium-mediated	Zmzb7	[27]
	Gene editing	UBI	U6	Agrobacterium-mediated	MYBR and AP2	[70]
	Genetic association	GOS2	GOS2	Particle-bombarded	ARGOS8	[71]
	Targeted mutagenesis	UBI	ZmU6	Agrobacterium-mediated	Argonaute 18	[72]
	Knock-out	-	U3	Agrobacterium-mediated	MS8	[28]
	Knock-out	35S	U3	Agrobacterium-mediated	zyp1	[73]
	Knock-out	ZmUBI	U6	Agrobacterium-mediated	ZmLG1	[74]
	Knock-out	UBI	U6	Particle bombardment	SDN1	[75]
	Targeted mutagenesis	UBI	U3 and U6	Agrobacterium-mediated	20 genes	[75]
	Gene editing	35SPPDK	U6	Agrobacterium-mediated	immature embryos	[76]
Sorghum	Functional genomics	Rice Actin 1	U6	Agrobacterium-mediated	DsRED2	[31]
	Gene editing	ZmUBI	U3	Agrobacterium-mediated	k1C	[77]
	Knock-out	ZmUBI	U3	Agrobacterium-mediated	PMI	[78]
	Gene editing	ZmUBI	U3	Particle bombardment	CAD and PDS	[79]
Barley	Knock-out	ZmUBI	U6	Agrobacterium-mediated	HvPM19	[80]
	Gene editing	ZmUBI	U6	Agrobacterium-mediated	hpt	[81]
	Fragment Deletions and Small Indels	ZmUBI	U6	Agrobacterium-mediated	ENGase	[29]
	Multiplex genome editing	ZmUBI	U6	Agrobacterium-mediated	HvCKX1	[82]
	Knock-out	ZmUBI	U3	Agrobacterium-mediated	HvMORC1	[30]
	Gene editing	CaMV 35s	U6	Agrobacterium-mediated	PDS1	[83]
	Functional studies	ZmUBI	-	Agrobacterium-mediated	dsRED	[84]
	Knock-out	ZmUBI	U6	Agrobacterium-mediated	hptII	[85]