Table 2: Central composite design for six independent variables and one response (ChOx concentration) with RSM and ANN predicted activity for ChOx (U/ml).
Run Order |
pH of Media |
Inoculum Age |
Inoculum Size |
Fermentation Period |
Temperature |
Shaking Speed |
ChOx Activity (U/ml) |
Experimental Observed |
RSM Predicted |
ANN Predicted |
1 |
7.5 |
48 |
11.25 |
-13.623 |
30 |
175 |
1.500 |
1.7608 |
1.50 |
2 |
7.5 |
48 |
11.25 |
72 |
30 |
175 |
3.890 |
4.0511 |
4.0617 |
3 |
7.5 |
48 |
11.25 |
72 |
30 |
175 |
4.040 |
4.0511 |
4.0617 |
4 |
9 |
66 |
7.5 |
108 |
20 |
250 |
3.040 |
2.8916 |
2.9653 |
5 |
6 |
66 |
15 |
36 |
20 |
100 |
2.800 |
2.6103 |
2.8000 |
6 |
9 |
30 |
15 |
36 |
20 |
100 |
2.770 |
2.6230 |
2.7701 |
7 |
6 |
30 |
15 |
108 |
40 |
250 |
1.540 |
1.5855 |
1.5399 |
8 |
9 |
30 |
15 |
108 |
40 |
100 |
2.150 |
2.1821 |
2.1500 |
9 |
7.5 |
48 |
11.25 |
72 |
30 |
175 |
4.150 |
4.0511 |
4.2117 |
10 |
6 |
66 |
15 |
108 |
20 |
250 |
1.700 |
1.9578 |
1.6999 |
11 |
7.5 |
48 |
11.25 |
72 |
53.7841 |
175 |
1.400 |
1.2402 |
1.4000 |
12 |
9 |
30 |
7.5 |
36 |
20 |
250 |
2.990 |
3.0401 |
2.9898 |
13 |
9 |
66 |
7.5 |
36 |
20 |
100 |
1.940 |
1.8341 |
1.4381 |
14 |
7.5 |
48 |
11.25 |
72 |
30 |
175 |
4.110 |
4.0511 |
4.0617 |
15 |
6 |
30 |
15 |
108 |
20 |
100 |
3.850 |
3.9209 |
3.8498 |
16 |
6 |
30 |
7.5 |
108 |
40 |
100 |
2.880 |
3.0573 |
2.8800 |
17 |
9 |
30 |
7.5 |
36 |
40 |
100 |
2.530 |
2.3934 |
2.5299 |
18 |
3.9324 |
48 |
11.25 |
72 |
30 |
175 |
1.750 |
1.6293 |
2.0265 |
19 |
9 |
66 |
7.5 |
36 |
40 |
250 |
2.910 |
2.7063 |
2.9099 |
20 |
6 |
30 |
15 |
36 |
40 |
100 |
2.240 |
2.3921 |
2.2400 |
21 |
6 |
30 |
7.5 |
108 |
20 |
250 |
3.500 |
3.4707 |
3.4999 |
22 |
7.5 |
90.8115 |
11.25 |
72 |
30 |
175 |
1.730 |
2.2532 |
1.2827 |
23 |
7.5 |
48 |
11.25 |
72 |
30 |
353.381 |
2.992 |
3.1280 |
3.0113 |
24 |
9 |
30 |
7.5 |
108 |
20 |
100 |
3.810 |
3.8522 |
3.2103 |
25 |
7.5 |
48 |
11.25 |
72 |
30 |
175 |
4.122 |
4.0511 |
4.0617 |
26 |
9 |
30 |
7.5 |
108 |
40 |
250 |
3.650 |
3.2969 |
2.9887 |
27 |
6 |
66 |
15 |
108 |
40 |
100 |
2.510 |
2.3804 |
2.5099 |
28 |
9 |
66 |
15 |
108 |
40 |
250 |
2.490 |
1.9286 |
2.4899 |
29 |
7.5 |
5.1885 |
11.25 |
72 |
30 |
175 |
3.670 |
3.4789 |
3.6699 |
30 |
7.5 |
48 |
11.25 |
72 |
30 |
175 |
4.116 |
4.0511 |
4.0617 |
31 |
6 |
66 |
7.5 |
36 |
40 |
100 |
2.500 |
2.6671 |
2.4999 |
32 |
6 |
30 |
7.5 |
36 |
40 |
250 |
2.150 |
1.6253 |
2.1499 |
33 |
6 |
66 |
7.5 |
36 |
20 |
250 |
1.790 |
1.6530 |
1.7899 |
34 |
6 |
66 |
7.5 |
108 |
40 |
250 |
1.942 |
2.1207 |
1.6703 |
35 |
7.5 |
48 |
11.25 |
72 |
6.2159 |
175 |
1.770 |
2.2435 |
1.7699 |
36 |
7.5 |
48 |
11.25 |
157.623 |
30 |
175 |
2.950 |
2.7959 |
2.950 |
37 |
9 |
66 |
15 |
108 |
20 |
100 |
1.850 |
1.9673 |
1.8501 |
38 |
9 |
66 |
15 |
36 |
20 |
250 |
2.130 |
1.9094 |
1.6292 |
39 |
11.067 |
48 |
11.25 |
72 |
30 |
175 |
0.740 |
1.8174 |
0.7400 |
40 |
7.5 |
48 |
20.1691 |
72 |
30 |
175 |
3.050 |
3.0884 |
2.6465 |
41 |
9 |
66 |
15 |
36 |
40 |
100 |
2.840 |
2.0986 |
2.8401 |
42 |
6 |
66 |
7.5 |
108 |
20 |
100 |
2.970 |
2.7009 |
2.9699 |
43 |
7.5 |
48 |
2.3309 |
72 |
30 |
175 |
3.440 |
4.0123 |
3.4400 |
44 |
7.5 |
48 |
11.25 |
72 |
30 |
-3.381 |
3.894 |
3.8647 |
3.8940 |
45 |
6 |
66 |
15 |
36 |
40 |
250 |
1.760 |
1.7025 |
1.7599 |
46 |
7.5 |
48 |
11.25 |
72 |
30 |
175 |
4.083 |
4.0511 |
4.0617 |
47 |
9 |
30 |
15 |
108 |
20 |
250 |
3.620 |
3.4204 |
3.6197 |
48 |
7.5 |
48 |
11.25 |
72 |
30 |
175 |
3.989 |
4.0511 |
4.0617 |
49 |
7.5 |
48 |
11.25 |
72 |
30 |
175 |
4.092 |
4.0511 |
4.0617 |
50 |
6 |
30 |
15 |
36 |
20 |
250 |
2.890 |
2.8138 |
2.8898 |
51 |
9 |
30 |
15 |
36 |
40 |
250 |
1.640 |
1.7400 |
1.6399 |
52 |
6 |
30 |
7.5 |
36 |
20 |
100 |
2.875 |
3.0498 |
2.8748 |
53 |
9 |
66 |
7.5 |
108 |
40 |
100 |
3.210 |
3.0892 |
3.6338 |