Table 1: Effect of organic solvents on the stability of immobilized and free HRP.

Chemicals Relative Activity (%)
Immobilized HRP Soluble HRP
Control 100 100
Isopropanol 107.79 35.63
Ethanol 155.27 57.33
Methanol 100.41 33.19
Acetonitrile 72.08 23.83
Triton X - 100 167.82 69.9
Tween - 20 142.5 55.24
Tween - 80 100.5 33.8