Fig. (2) Phylogenetic tree of ISA enzymes. The tree is based on the alignment of OsttaISA1 with the amino acid sequences obtained with PSI-Blast. The tree was built by Maximum Parsimony method in MEGA X version 10.0.4 (49). Phylogenies were determined by Bootstrap Analysis of 500 replicates in MEGA X. Branch lengths are proportional to distances. Bootstrap values are shown above branches.