Table 1: List of residues involved in catalysis and/or binding.
| Residue |
Function |
Conserved Function |
| Asp363 (Trex) |
catalytic nucleophyle |
YES (Asp466) |
| Glu399 (Trex) |
catalytic acid/base |
YES (Glu526) |
| Asp471 (Trex) |
transition state stabilizer |
YES (Asp597) |
| Trp757 (ChlreISA1) |
stacking interactions SBS1 |
YES (Trp740) |
| Trp767 (ChlreISA1) |
stacking interactions SBS1 |
YES (Trp750) |
| Trp652 (ChlreISA1) |
stacking interactions SBS2 |
YES (Trp629) |
| His794 (ChlreISA1) |
stacking interactions SBS2 |
YES (His778) |
| Trp856 (ChlreISA1) |
stacking interactions SBS2 |
NO (Arg840) |
| Arg667 (ChlreISA1) |
key for dimerization |
NO (Ala648) |
| Asp848 (ChlreISA1) |
key for dimerization |
NO (Lys832) |
Ref: Trex: Sulfolobus solfataricus glycogen debranching enzyme; ChlreISA1: Chlamydomonas reinhardtii isoamylase; SBS1: carbohydrate surface binding site 1; SBS2: carbohydrate surface binding site 2.