Table 1: Primer sequences, temperatures (Tm), lengths, amplicon size for each antioxidant gene. Glutathione peroxidase 1(Gpx1), Peroxiredoxin 3 (Prdx3), Superoxide dismutase 1 (SOD 1), Catalase (CAT), and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
| No. |
Primer |
Sequence 5′–3′ |
Tm |
Length |
Amplicon Size |
| 1 |
Gpx1_forward |
ATTGCCTCCAAATACAACCTC |
50.3 °C |
21bp |
408bp |
| Gpx1_reverse |
TGCGTCATCAAAAGGCTTC |
50.9 °C |
19bp |
| 2 |
Prdx3_forward |
AGTATTTCTGCCTCAACAGTTC |
50.5 °C |
22bp |
399bp |
| Prdx3_reverse |
GCCCAAACCACCATTCTTTC |
51.8 °C |
20bp |
| 3 |
SOD 1_forward |
ACTTCGAGCAGAAGGCAAG |
52.1 °C |
19bp |
458bp |
| SOD 1_reverse |
GCAATCCCAATCACTCCAC |
50.6 °C |
19bp |
| 4 |
CAT_forward |
ACAACACACATACCACACAC |
50.5 °C |
20bp |
392bp |
| CAT_reverse |
GCTGAAAGCAACCAAACAC |
50.3 °C |
19bp |
| 5 |
GAPDH_forward |
CCCCTTCATTGACCTCAACTAC |
52.5 °C |
22bp |
420bp |
| GAPDH_reverse |
ATGAGCCCTTCCACAATGC |
52.3 °C |
19bp |