Table 2: Functional classification of proteins detected in the secretome of T. cellulosilytica TB100 using RS as complex substrate.

Description/ Protein Name Uniprot
Accession
NO.
MW
(kDa)
Coverage% Peptides Matched Amino Acid No. Protein
Family
PI CBM Signal Peptide
Cellulases
Endoglucanase
Cellulose 1,4-beta-cellobiosidase
Glucanase
Endoglucanase
Glycoside Glycoside hydrolase
Endoglucanase
Endoglucanase
Glucanase

A0A147KLC2
A0A147KIK5
A0A147KI07
A0A0A7A855
A0A147KHU4
A0A147KHF1
A0A147KMV8
A0A147KIV9

68.3
106.3
46.6
46.2
100.8
103.8
102.4
61.9

5.02
15.9
17.4
4.78
1.59
16.17
17.45
34.69

2
16
11
3
1
14
15
17

618
981
454
439
941
965
957
588

Cellulase
GH48
GH6
Cellulase
GH81
GH9
GH9
GH6

4.51
4.35
5.77
4.71
5.03
4.67
4.14
4.30

CBM3
CBM2
CBM2
CBM2
-
CBM2,3
CBM2
CBM2

Y
Y
N
Y
Y
N
N
N
Hemicellulases
Beta-xylanase
Beta-xylanase
Xyloglucanase
Xylose isomerase
Beta-xylosidase

A0A147KDP3
A0A147KJ27
A0A147KMS2
A0A147KE49
A0A147KMT4

53.4
50.5
97.8
43.1
61.8

11.02
21.1
2.29
3.12
2.18

8
8
5
1
3

490
474
919
385
550

GH10
GH10
-
AP_endonuc_2
GH43

5.41
4.49
4.29
5.09
5.88

CBM2
CBM2
CBM2
-
-

Y
Y
Y
N
N
Proteases
Aminopeptidase Y
Peptidase S1
ATP-dependent Clp protease ATP-binding subunit ClpX

A0A147KFZ9
A0A147KH40
A0A147KHB4

53.8
38.2
46.9

8.74
2.95
3.3

6
1
1

515
373
424

Peptidase_M28
Peptidase S1
AAA

5
4.48
5.03

-
-
-

Y
Y
N
Others
Glutamyl-tRNA amidotransferase Uncharacterized protein Uncharacterized protein
Peptide ABC transporter substrate-binding protein
Growth inhibitor PemK
Glycine/betaine ABC transporter substrate-binding protein
Dihydrolipoyl dehydrogenase
Surface protein
Glutamate-binding protein
Branched-chain amino acid ABC transporter substrate-binding protein
Cell division protein DivIVA
Cellulose-binding protein
Protein GrpE
50S ribosomal protein L11
RNA helicase [Fragment]

A0A147KH75
A0A147KDP0 A0A147KM68
A0A147KIK0

A0A147KJW7
A0A147KDQ0

A0A147KD21
A0A147KIW2
A0A147KMF2
A0A147KMP9

A0A147KL19
A0A147KKN7
A0A147KM11
A0A147KL98
A0A147KIV8

16.3
22.5
27.2
63.5

17.3
33.9

48.3
39.2
29.7
42.2

31.6
25.0
27.7
15.0
89.5

6.67
9.05
4.38
8.86

5.33
10.19

10.24
2.34
11.58
1.93

6.71
4.05
3.49
9.86
1.24

1
1
1
6

1
2

5
1
3
1

2
1
1
1
1

150
210
251
587

150
314
459

384
285
414

283
222
258
142
804

-
-
HTH GntR

SBP_bac_5
PemK_toxin
OpuAC

Pyr_redox_2
TED
SBP_bac_3
PBP6

DivIVA
LPMO10
GrpE
Ribosomal_L11
rRNA_proc_
arch

6.43
11.15
6.02
4.08

5.37
4.02

5.44
4.34
4.06
3.52

4.96
8.40
4.32
9.10
9.02

-
-
-
-

-
-

-
-
-
-

-
-
-
-
-

N
N
N
Y

N
Y

N
Y
Y
N

N
Y
N
N
N