Fig. (3a) SDS-PAGE (20% Acrylamide and Tris/Tricine Buffer) Ni-NTA column purification of 5 kDa protein from the clone carrying recombinant pBAD C(Lane 1 - Protein Molecular mass marker; Lane 2 - Cell Lysate; Lane 3 - Flow-through; Lane 4 - First wash; Lane 5 - Second wash; Lane 6 - First Eluate (concentrated); Lane 7 - Second Eluate (dilute); Arrow indicates the 5 kDa protein). The image shows that the 5kDa protein was able to be successfully expressed from the recombinant pBAD vector in the transformed E. coli cells.