Table 1:
The reaction mix was set as follows to check the antithrombotic activity.
Component
Test Volume (µL)
Control Volume (µL)
Platelet Suspension
50
50
Pepsin Digested Fragments
50
n.d.
Elution Buffer E
n.d.
50
Fibrinogen
(
γ
=0.75 mg/mL)
10
10
ADP (inducer
c
=5 µM)
5μM Final Concentration
1
1
Total
111
111
n.d. = not determined.