Fig. (3) SDS-PAGE and rpLF identification in various purification steps. A) Coomassie blue-stained SDS–PAGE for crude extraction (20 μg, lane 1), supernatants of 4% ammonium sulfate treatment (20 μg, lane 2), precipitates of 55% ammonium sulfate treatment (20 μg, lane 3), and column-purified rpLF (1 μg, lane 4). The molecular weight markers are indicated in kDa. B) Western blot analysis of rpLF with the same amount of protein (20 μg) as above, except 0.01 μg of column-purified rpLF (lane 4) was used.