Fig. (5) Molecular mass and glycosylated isoform identification of purified rpLF using MALDI-TOF and PNGase treatment. A) Purified rpLF was subjected to MALDI-TOF, and two peaks with molecular masses of 76.7 and 77.3 kDa were identified. B) Coomassie blue-stained SDS–PAGE of purified rpLF (1 μg) and rpLF, which were incubated with PNGase F at 37°C for 2 hours (rpLF+PNGase F). The molecular weight markers are indicated in kDa.