Table 1: Purification of recombinant lactoferrin from transgenic rice seedsa.
| Steps |
Total protein
(mg)b
|
rpLF
(µg)c
|
Recovery
(%) |
Purity
(%)d
|
| 1. Crude extract |
815 |
987 |
100 |
0.12 |
| 2. Supernatant of 4% ammonium sulfate |
734 |
972 |
98.5 |
0.13 |
| 3. Precipitate of 55% ammonium sulfate |
97 |
754 |
76.4 |
0.78 |
| 4. Heparin-Sepharose column |
326x10-3
|
326 |
33.0 |
100 |
a One hundred grams of transgenic rice seeds was used
b The concentration of protein was determined by the Bradford method
c The concentration of protein was determined by ELISA
d The purity of rpLF was measured by dividing c over b