Fig. (2) HDAC2 is a cytoplasmic protein in differentiating dermal skin hair follicles and tongue filiform papillae. (A) Low magnification image of cytoplasmic HDAC2 in developing P2 dermal hair follicles. (B) HDAC2 immunoreactivity merged with the nuclear dye DAPI. (C) High magnification DAPI merged image of P2 hair follicle demonstrating cytoplasmic HDAC2 restriction to the dermal sheath cells. (D) Low magnification image of HDAC2 expression in a spatially restricted population of filiform papillae on the dorsal tongue surface P2 mice. (E) HDAC2 tongue immunoreactivity merged with the nuclear dye DAPI. (F) High magnification DAPI merged image of HDAC2 positive filiform papillae demonstrating cytoplasmic HDAC2. (G) Subcellular fractionation of representative epithelial tissue culture cells (C4-2) and in vivo E17 murine epidermal cells confirms HDAC2 cytoplasmic localization in murine epidermis. 14-3-3β serves as a marker of cytoplasmic fraction while histone H3 and p63 serve as nuclear markers for c4-2 cells and murine skin, respectively.