Table 2.: Primer Sequences Used for the PCR Multiplex, PCR-RFLP and the SBE Reaction

Polymorphic Site PCR Primer Position (*) SNP SBE Primer Position (*)
7025 5´-CTGACTGGCATTGTATTAGCA-3´ 6960F C7028T 5´-ACACGACACGTACTACGTTGTAGC-3´ 7004F
5´-GTATACGGGTTCTTCGAATG-3´ 7433R
14766 5´-GAGAAGGCTTAGAAGAAAACCCCAC-3´ 14601F T14766C 5'-cgatcATGAGTGGTTAATTAATTTTATTA-GGGGGTTA-3' 14798R
5´-GTGGGCGATTGATGAAAAGGC-3´ 14950R
10394 5´-GGCCTATGAGTGACTACAAAAA-3´ 10364F A10398G 5'-ataTATGAGTGACTACAAAAAGGATTAGACTGA-3' 10368F
5´-TATTCCTAGAAGTGAGATGGT-3´ 10526R
4577 5´-CCTACCACTCACCCTAGCATTAC-3´ 4185F G4580A 5'-(at)7TTTTTTACCTGAGTAGGCCTAGAAATAAACAT-3' 4548F
5´-TAGGAATGCGGTAGTAGTTAG-3´ 5120R
12308 5´-CAACCCCGACATCATTACCGGGT-3´ 12106F A12308G 5'-(tacg)5aCCATTGGTCTTAGGCCCCAA-3' 12288F
5´-GGGTTAACGAGGGTGGTAAGG-3´ 12413R
4216 5´-CCTACCACTCACCCTAGCATTAC-3´ 4185F T4216C 5´-cgCCACTCACCCTAGCATTACTTATATGA-3´ 4189F
5´-GCGAGCTTAGCGCTGTGATGAG-3´ 4542R
10032(**) 5´-CTTTGGCTTCGAAGCCGCCGCC-3´ 9902F A10029G
5´-TATTCCTAGAAGTGAGATGGT-3´ 10526R
14465 5´-ATGCCTCAGGATACTCCTCAATAGCCATC-3´ 14430F T14470C
5´-CCGTGCGAGAATAATGATGTATGC-3´ 14686R
8994(**) 5´-TAGCCCACTTCTTACCACAAGGC-3´ 8900F G8994A
5´-GTGTGAAAACGTAGGCTTG-3´ 9172R

Lower case letters indicate the unspecific nucleotides in 5´-end of the SBE primer. (*) R: primer in reverse orientation; F: primer in forward orientation.
(**) Digested PCR products will appear like three fragments in an agarose gel, whereas undigested products like two fragments.