Fig. (1) LET-756 expression in wild type and let-756R318 transformed worms. (A) In this mosaic worm, GFP driven from the let-756 promoter is expressed in body muscles (bm), pharynx procorpus (ph), cephalic neurons (cn), CAN cells (can), and G1 and G2 gland cells (gc). (B – E) worms were transformed by Plet-756::let-756::gfp, fixed and immunostained with anti-GFP antiserum. Accumulation of fluorescence is observed in a punctuate pattern at the junctions between body muscle cells (C) and around the pharynx (D). CAN cells are stained (E). (F) worms were transformed by Plet-756::let-756R318::gfp; the construct was mainly expressed in neurons, which were identified as the ASE chemosensorial neurons since they do not fill with the lipophilic DiI dye and exhibit typical endings (magnified in the right insert).