Table 1: Design matrix and citric acid yield obtained after the fermentation process.
- |
Temperature |
Time |
pH |
Methanol |
Glucose |
Observed citric |
Predicted citric |
Residuals |
Run order |
oC |
h |
|
conc (g/L) |
conc (g/L) |
acid yield (g/L) |
acid yield (g/L) |
(g/L) |
1 |
25 |
10 |
5 |
24 |
3 |
12.33 |
12.30 |
0.03 |
2 |
30 |
40 |
6 |
60 |
4 |
11.77 |
11.73 |
0.04 |
3 |
30 |
40 |
3 |
60 |
7 |
12.20 |
11.77 |
0.43 |
4 |
30 |
110 |
3 |
60 |
4 |
10.36 |
10.19 |
0.17 |
5 |
30 |
30 |
3 |
60 |
4 |
10.28 |
10.21 |
0.07 |
6 |
40 |
40 |
3 |
60 |
4 |
13.18 |
11.91 |
1.27 |
7 |
25 |
80 |
2 |
96 |
6 |
12.97 |
12.71 |
0.26 |
8 |
25 |
10 |
2 |
96 |
3 |
13.28 |
13.50 |
-0.22 |
9 |
25 |
10 |
2 |
24 |
6 |
10.31 |
10.40 |
-0.10 |
10 |
35 |
80 |
5 |
96 |
6 |
10.23 |
9.73 |
0.50 |
11 |
25 |
80 |
5 |
24 |
6 |
12.38 |
12.31 |
0.07 |
12 |
30 |
40 |
0 |
60 |
4 |
12.10 |
12.12 |
-0.02 |
13 |
35 |
80 |
2 |
24 |
6 |
11.20 |
11.35 |
-0.15 |
14 |
25 |
80 |
2 |
24 |
3 |
14.29 |
14.05 |
0.25 |
15 |
30 |
40 |
3 |
60 |
1 |
12.66 |
12.42 |
0.24 |
16 |
35 |
10 |
2 |
24 |
3 |
11.69 |
11.77 |
-0.08 |
17 |
30 |
40 |
3 |
72 |
4 |
15.51 |
15.54 |
-0.03 |
18 |
35 |
80 |
2 |
96 |
3 |
12.74 |
12.87 |
-0.13 |
19 |
25 |
10 |
5 |
96 |
6 |
10.08 |
11.74 |
-1.67 |
20 |
20 |
40 |
3 |
60 |
4 |
10.26 |
10.55 |
-0.29 |
21 |
35 |
10 |
5 |
96 |
3 |
10.36 |
10.12 |
0.24 |
22 |
30 |
40 |
3 |
12 |
4 |
11.13 |
10.87 |
0.26 |
23 |
35 |
10 |
2 |
96 |
6 |
10.92 |
11.11 |
-0.18 |
24 |
30 |
40 |
3 |
60 |
4 |
10.46 |
11.08 |
-0.62 |
25 |
25 |
80 |
5 |
96 |
3 |
10.33 |
10.44 |
-0.11 |
26 |
35 |
10 |
5 |
24 |
6 |
13.02 |
13.06 |
-0.04 |
27 |
35 |
80 |
5 |
24 |
3 |
10.49 |
10.67 |
-0.18 |