Fig. (2) Segmentation of a typical cine stack of a rat heart. The red line delineates the endocardial border, whilst the green line marks the epicardial border. The top and bottom rows depict the end diastolic (ED) and end systolic (ES) time frames, respectively. The columns show different slices where the most apical slice included is labelled as slice 1 (S01). Parameters; in-plane resolution: 200x200 µm, slice thickness 1 mm, RV: right ventricle, LV: left ventricle.