Fig. (1) Histological tissue sections: A) non-biotin labeled heart; control. B) and C) biotin perfused heart. Biotinylation; brown color, is restricted to the coronary vascular endothelium. All capillaries were labeled by the biotin (arrows). D) Electrophoretic patterns of CM protein fraction during control (C), I and I/R. E) Electrophoretic patterns of biotinylated coronary VELM. CM protein fractions from SDS-PAGE were transferred to nitrocellulose membranes and coronary VELM proteins revealed by DAB. I/R patterns (E) display more bands of low molecular weight proteins compared to C.
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