Table 1: Effect of Pertussis Toxin (PTX) on Niacin (NA)-mediated Decrease of Stimulated Cytokine Secretion

Cytokine Mice Condition Treatment Cytokine Levels (pg/ml)
IL-1β Normal         AGD LPS
LPS+NA
LPS+NA+PTX
  LPS
LPS+NA
LPS+NA+PTX
3194 ± 303 
2322 ± 267**
2995 ± 298#
  3854 ± 321
2256 ± 287**
3764 ± 333#
  TNF-α   Normal       AGD   LPS
LPS+NA
LPS+NA+PTX
  LPS
LPS+NA
LPS+NA+PTX
  3546 ± 294
2123 ± 189**
3721 ± 311#
  4247 ± 395
2233 ± 227**
4323 ± 344#
  IL-6   Normal       AGD   LPS
LPS+NA
LPS+NA+PTX
  LPS
LPS+NA
LPS+NA+PTX
  15442 ± 1670
10456 ± 998**
16028 ± 1687#
  21234 ± 1711
10786 ± 1213**
19999 ± 1532#

Macrophages from control or AGD mice were pretreated with vehicle or PTX (50 ng/ml) for 18 h. After this time cells were incubated in the presence or absence of 1000 mM of Niacin (NA) and stimulated with LPS (10mg/ml) + IFN-g (100 U/ml) for 24 h. Cytokine levels were determined as described in Fig (4). Data are mean ± S.E.M. of three experiments in each group. **p<0.01 vs the corresponding basal value, # p<0.05 respect NA value.