Table 1: Amount of Calcium Ions released (in mg/L) according to agitation protocol and chelating agent employed.

Chelating agent
Agitation protocol Distilled water 0.2% Chitosan 17% EDTA
Manual 1.52 ± 0.07Aa 161.96 ± 2.18Ab 25.10 ± 3.15Ac
Sonic 2.55 ± 0.35Ba 166.94 ± 2.19Bb 64.95 ± 2.74Bc
Ultrasonic 3.49 ± 0.13Ca 176.24 ± 2.80Cb 85.74 ± 3.45Cc

Data presented as means ± standard deviation.
* Different upper-case letters indicate statistically significant differences (P<0.05) within columns.
* Different lower-case letters indicate statistically significant differences (P<0.05) within rows.