Fig. (2) MSE suppressed IL-6 (a) and IL-8 (b) mRNA expressionin hPDL fibroblasts. Cells were incubated with 1.25, 2.5 and 5.0 µg/ml MSE or 0.05% DMSO for 1 h, followed by 10 μg/ml P. gingivalis LPS stimulation. After 24 h, cells were extracted for Real-time PCR. Levels of IL-6 and IL-8 mRNA were normalized to GAPDH. The values (mean ± SD) are the average of three independent experiments performed in triplicate; *indicate significant difference from P. gingivalis LPS treatment only (p < 0.05).