Table 1: pH cycling treatment sequence for the experiment.

Time Treatment
Day 1 is all-day storage in remineralization solution. Then, subsequent days’ treatments will be as follows
2 min (starts 8:00 am)
Approximately 1 hr to complete all groups.
Toothpaste treatment
Rinse with deionized distilled water
6 hr (9:00 am – 3:00 pm) Acid challenge (Demineralization)
Rinse with deionized distilled water
2 min (starts 3:00 pm)
Approximately 1 hr to complete all groups.
Toothpaste treatment
Rinse with deionized distilled water
16 hrs (From 4:00 pm till 8:00 am next day) Storage in Remineralization solution
Repeat for 13 additional Days (Human)

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