| Time | Treatment |
|---|---|
| Day 1 is all-day storage in remineralization solution. Then, subsequent days’ treatments will be as follows | |
| 2 min (starts 8:00 am) Approximately 1 hr to complete all groups. |
Toothpaste treatment |
| Rinse with deionized distilled water | |
| 6 hr (9:00 am – 3:00 pm) | Acid challenge (Demineralization) |
| Rinse with deionized distilled water | |
| 2 min (starts 3:00 pm) Approximately 1 hr to complete all groups. |
Toothpaste treatment |
| Rinse with deionized distilled water | |
| 16 hrs (From 4:00 pm till 8:00 am next day) | Storage in Remineralization solution |
| Repeat for 13 additional Days (Human) | |