Fig. (5) The β-galactosidase activity is retained at 42°C but not at 50°C.β-galactosidase and von Kossa staining of the cross-sections of 3-month-old mouse incisors decalcified at RT (a, b), 37°C (c, d), 42°C (e, f), and 50°C (g, h). The β-galactosidase enzyme is active in teeth decalcified at RT, 37°C and 42°C, but not at 50°C, as indicated by the blue staining. 0.1M EDTA is used as a demineralizing agent for all of the temperatures tested. The plastic section of the undecalcified incisor that is the same age as the control slide for von Kossa staining (i).