Fig. (5) Effects of CHX and NaF on biofilm bacterial vitality. Biofilms were treated with (a) 0.1% CHX or (b) 0.2% NaF in situ for 1 minute prior to staining with BacLight Live/Dead. Z-plane sections were made and used to generate sagital sections through the biofilms. To allow assessment of the vitality of bacterial at different levels, the number of green and red pixels in each section was plotted against biofilm depth.