Fig. (3) CLSM images of F. nucleatum (A, E) T. forsythia (B, F) V. atypica (C, G) K. pneumoniae (D, H) biofilms formed on DBR disc
surfaces after treatment with Polident® experimental formulation M138-12 (upper panel) and Efferdent® (lower panel), respectively. Denture
biofilms were stained with SYTO59 and SYTOX Green as described in Materials and Methods, and examined by CLSM to reveal the live
(green) and dead (red) cell population following treatment. Four random fields of view were examined for each sample and representative
images taken through a 20x objective are shown (Scale bar, 50 μm).