Fig. (3) CLSM images of F. nucleatum (A, E) T. forsythia (B, F) V. atypica (C, G) K. pneumoniae (D, H) biofilms formed on DBR disc surfaces after treatment with Polident® experimental formulation M138-12 (upper panel) and Efferdent® (lower panel), respectively. Denture biofilms were stained with SYTO59 and SYTOX Green as described in Materials and Methods, and examined by CLSM to reveal the live (green) and dead (red) cell population following treatment. Four random fields of view were examined for each sample and representative images taken through a 20x objective are shown (Scale bar, 50 μm).

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